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cd29 miltenyi  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd29 miltenyi
    Cd29 Miltenyi, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd29+fitc/CD90+Antibody%2C+anti-human%2C+FITC/pmc10881830__DataSheet1-2-20-21
    Average 95 stars, based on 74 article reviews
    cd29 miltenyi - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    FACS:

    Article Title: Laminin matrix adhesion regulates basal mammary epithelial cell identity.
    Article Snippet: The cell pellet was resuspended in MMEC growth medium [advanced DMEM/F12 containing 5 μg/ml insulin, 1 μg/ml hydrocortisone, 10 ng/ml mouse EGF, 2 mM glutamine, 50 μg/ml gentamycin and penicillin and streptomycin (all from Sigma) supplemented with 10% FCS (Gibco)] for further experiments or cells were subjected to FACS sorting for separating basal and luminal MMECs (see below). .. FACS sorting of primary MMECs Single-cell suspensions of isolated primary MMECs were resuspended in 0.2% BSA in Dulbecco’s PBS and the cells were incubated with either of the following sets of primary antibodies: CD31–PE, CD45–PE, Ter119–PE, CD24–Pacific Blue (fromBectonDickinson) and CD29–FITC (fromSigma), or CD29–FITC (Miltenyi Biotec, 130-102-975), CD326 (Ep-CAM) BV786 (BD; 740958), CD45 PerCP–Cy5.5 (Tonbo Biosciences; 65-0452-U100), CD31 PerCP–Cy5.5 (BD; 562861) and Ter-119 PerCP-Cy5.5 (BD; 560512). ..

    Single Cell:

    Article Title: Laminin matrix adhesion regulates basal mammary epithelial cell identity.
    Article Snippet: The cell pellet was resuspended in MMEC growth medium [advanced DMEM/F12 containing 5 μg/ml insulin, 1 μg/ml hydrocortisone, 10 ng/ml mouse EGF, 2 mM glutamine, 50 μg/ml gentamycin and penicillin and streptomycin (all from Sigma) supplemented with 10% FCS (Gibco)] for further experiments or cells were subjected to FACS sorting for separating basal and luminal MMECs (see below). .. FACS sorting of primary MMECs Single-cell suspensions of isolated primary MMECs were resuspended in 0.2% BSA in Dulbecco’s PBS and the cells were incubated with either of the following sets of primary antibodies: CD31–PE, CD45–PE, Ter119–PE, CD24–Pacific Blue (fromBectonDickinson) and CD29–FITC (fromSigma), or CD29–FITC (Miltenyi Biotec, 130-102-975), CD326 (Ep-CAM) BV786 (BD; 740958), CD45 PerCP–Cy5.5 (Tonbo Biosciences; 65-0452-U100), CD31 PerCP–Cy5.5 (BD; 562861) and Ter-119 PerCP-Cy5.5 (BD; 560512). ..

    Article Title: Laminin alpha 5 regulates mammary gland remodeling through luminal cell differentiation and Wnt4-mediated epithelial crosstalk
    Article Snippet: .. Single-cell suspension of isolated primary mouse mammary epithelial cells (MMECs) was resuspended in 0.2% BSA in Dulbecco's PBS, and the cells were incubated with the following primary antibodies for analysis: CD29-FITC (Miltenyi Biotech, 130-102-975); CD24-APC (M1/69, BioLegend, 101814); CD31-BV421 (MEC13.3, BD Biosciences, 562939); CD45-BV421 (30 F11, BD Biosciences, 563890); Ter119-BV421 (BD Biosciences, 563998); Sca1-BV711 (D7, BioLegend, 108131); and CD49b-PE (HMα2, BioLegend, 103506). ..

    Isolation:

    Article Title: Laminin matrix adhesion regulates basal mammary epithelial cell identity.
    Article Snippet: The cell pellet was resuspended in MMEC growth medium [advanced DMEM/F12 containing 5 μg/ml insulin, 1 μg/ml hydrocortisone, 10 ng/ml mouse EGF, 2 mM glutamine, 50 μg/ml gentamycin and penicillin and streptomycin (all from Sigma) supplemented with 10% FCS (Gibco)] for further experiments or cells were subjected to FACS sorting for separating basal and luminal MMECs (see below). .. FACS sorting of primary MMECs Single-cell suspensions of isolated primary MMECs were resuspended in 0.2% BSA in Dulbecco’s PBS and the cells were incubated with either of the following sets of primary antibodies: CD31–PE, CD45–PE, Ter119–PE, CD24–Pacific Blue (fromBectonDickinson) and CD29–FITC (fromSigma), or CD29–FITC (Miltenyi Biotec, 130-102-975), CD326 (Ep-CAM) BV786 (BD; 740958), CD45 PerCP–Cy5.5 (Tonbo Biosciences; 65-0452-U100), CD31 PerCP–Cy5.5 (BD; 562861) and Ter-119 PerCP-Cy5.5 (BD; 560512). ..

    Article Title: Laminin alpha 5 regulates mammary gland remodeling through luminal cell differentiation and Wnt4-mediated epithelial crosstalk
    Article Snippet: .. Single-cell suspension of isolated primary mouse mammary epithelial cells (MMECs) was resuspended in 0.2% BSA in Dulbecco's PBS, and the cells were incubated with the following primary antibodies for analysis: CD29-FITC (Miltenyi Biotech, 130-102-975); CD24-APC (M1/69, BioLegend, 101814); CD31-BV421 (MEC13.3, BD Biosciences, 562939); CD45-BV421 (30 F11, BD Biosciences, 563890); Ter119-BV421 (BD Biosciences, 563998); Sca1-BV711 (D7, BioLegend, 108131); and CD49b-PE (HMα2, BioLegend, 103506). ..

    Incubation:

    Article Title: Laminin matrix adhesion regulates basal mammary epithelial cell identity.
    Article Snippet: The cell pellet was resuspended in MMEC growth medium [advanced DMEM/F12 containing 5 μg/ml insulin, 1 μg/ml hydrocortisone, 10 ng/ml mouse EGF, 2 mM glutamine, 50 μg/ml gentamycin and penicillin and streptomycin (all from Sigma) supplemented with 10% FCS (Gibco)] for further experiments or cells were subjected to FACS sorting for separating basal and luminal MMECs (see below). .. FACS sorting of primary MMECs Single-cell suspensions of isolated primary MMECs were resuspended in 0.2% BSA in Dulbecco’s PBS and the cells were incubated with either of the following sets of primary antibodies: CD31–PE, CD45–PE, Ter119–PE, CD24–Pacific Blue (fromBectonDickinson) and CD29–FITC (fromSigma), or CD29–FITC (Miltenyi Biotec, 130-102-975), CD326 (Ep-CAM) BV786 (BD; 740958), CD45 PerCP–Cy5.5 (Tonbo Biosciences; 65-0452-U100), CD31 PerCP–Cy5.5 (BD; 562861) and Ter-119 PerCP-Cy5.5 (BD; 560512). ..

    Article Title: Synergistic Effect of Human Chorionic Gonadotropin and Granulocyte Colony Stimulating Factor in the Mobilization of HSPCs Improves Overall Survival After PBSCT in a Preclinical Murine Model. Are We Far Enough for Therapy?
    Article Snippet: Strategies to improve hematopoietic stem and progenitor cell (HSPC) mobilization from the bone marrow can have a pivotal role in addressing iatrogenic bone-marrow insufficiency from chemo(radio)therapy and overcoming peripheral blood stem cell transplantation (PBSCT) limitations such as insufficient mobilization.. Granulocyte-colony stimulating factor (G-CSF) represents the standard mobilization strategy for HSPC and has done so for more than three decades since its FDA approval.. Its association with non–G-CSF agents is often employed for difficult HSPC mobilization.

    Article Title: Laminin alpha 5 regulates mammary gland remodeling through luminal cell differentiation and Wnt4-mediated epithelial crosstalk
    Article Snippet: .. Single-cell suspension of isolated primary mouse mammary epithelial cells (MMECs) was resuspended in 0.2% BSA in Dulbecco's PBS, and the cells were incubated with the following primary antibodies for analysis: CD29-FITC (Miltenyi Biotech, 130-102-975); CD24-APC (M1/69, BioLegend, 101814); CD31-BV421 (MEC13.3, BD Biosciences, 562939); CD45-BV421 (30 F11, BD Biosciences, 563890); Ter119-BV421 (BD Biosciences, 563998); Sca1-BV711 (D7, BioLegend, 108131); and CD49b-PE (HMα2, BioLegend, 103506). ..

    Staining:

    Article Title: Glucose-6-phosphate-dehydrogenase on old peroxisomes maintains self-renewal of epithelial stem cells after asymmetric cell division.
    Article Snippet: Nature Communications | (2025) 16:3932 7 treated with 0.05% Trypsin-EDTA (DIFCO, J.T.BAKER) for 7-10minutes, the cell suspension was filter through a 70 μm cell strainer and centrifuged at 300xg for 5minutes. .. The cell pellet was stained with the following antibodies: CD29 FITC (Miltenyi Biotec, 130-102-975), CD326 (Ep-CAM) BV786 (BD; 740958), CD45 PerCP-Cy5.5 (Tonbo Biosciences; 65-0452-U100), CD31 PerCP-Cy5.5 (BD; 562861), Ter-119 PerCP-Cy5.5 (BD; 560512) for 30minutes on ice (1:500 dilution for all antibodies). .. After washing with Advance DMEM/F12 base media, cells were resuspended in mMEC growth media with 7AAD (Life Technologies, A1310, 1 μg/ml) for at least 5minutes on ice before sorting.

    Article Title: Glucose-6-phosphate-dehydrogenase on old peroxisomes maintains self-renewal of epithelial stem cells after asymmetric cell division
    Article Snippet: Next, cell pellets were treated with 0.05% Trypsin-EDTA (DIFCO, J.T.BAKER) for 7-10 minutes, the cell suspension was filter through a 70 μm cell strainer and centrifuged at 300xg for 5 minutes. .. The cell pellet was stained with the following antibodies: CD29 FITC (Miltenyi Biotec, 130-102-975), CD326 (Ep-CAM) BV786 (BD; 740958), CD45 PerCP-Cy5.5 (Tonbo Biosciences; 65-0452-U100), CD31 PerCP-Cy5.5 (BD; 562861), Ter-119 PerCP-Cy5.5 (BD; 560512) for 30 minutes on ice (1:500 dilution for all antibodies). .. After washing with Advance DMEM/F12 base media, cells were resuspended in mMEC growth media with 7AAD (Life Technologies, A1310, 1 μg/ml) for at least 5 minutes on ice before sorting.

    Labeling:

    Article Title: 3D Printed Integrated Bionic Oxygenated Scaffold for Bone Regeneration.
    Article Snippet: The repair of large bone defects remains a challenging problem in bone tissue engineering.. Ischemia and hypoxia in the bone defect area make it difficult for seed cells to survive and differentiate, which fail to perform effective tissue regeneration.. Current oxygen-producing materials frequently encounter problems such as a rapid degradation rate, insufficient mechanical properties, difficult molding, and cumbersome fabrication.

    Flow Cytometry:

    Article Title: Hsa-miR-532-3p protects human decidual mesenchymal stem cells from oxidative stress in recurrent spontaneous abortion via targeting KEAP1
    Article Snippet: .. The following markers of hDMSCs were detected by flow cytometry (FC) (BDLSRFortessa): CD3-FITC (1:100, Southern Biotechnology, 9515-02S), CD29-FITC(1:100, Miltenyi, 130-123-692), CD34-FITC(1:100, Miltenyi, 130-113-740), CD44-FITC(1:100, Miltenyi, 130-124-856), CD45-FITC(1:100, Miltenyi, 130-110-631), CD73-FITC (1:100, TRAN, HF101-01), CD90-FITC (1:100, Miltenyi, 130-114-901), and CD105-FITC (1:100, Miltenyi, 130-112-169). ..

    Article Title: Hsa-miR-532-3p protects human decidual mesenchymal stem cells from oxidative stress in recurrent spontaneous abortion via targeting KEAP1.
    Article Snippet: .. The following markers of hDMSCs were detected by flow cytometry (FC) (BDLSRFortessa): CD3-FITC (1:100, Southern Biotechnology, 9515-02S), CD29-FITC(1:100, Miltenyi, 130-123-692), CD34-FITC (1:100, Miltenyi, 130-113-740), CD44-FITC(1:100, Miltenyi, 130-124- 856), CD45-FITC(1:100, Miltenyi, 130-110-631), CD73-FITC (1:100, TRAN, HF101-01), CD90-FITC (1:100, Miltenyi, 130-114-901), and CD105-FITC (1:100, Miltenyi, 130-112-169). ..

    Clinical Proteomics:

    Article Title: Synergistic Effect of Human Chorionic Gonadotropin and Granulocyte Colony Stimulating Factor in the Mobilization of HSPCs Improves Overall Survival After PBSCT in a Preclinical Murine Model. Are We Far Enough for Therapy?
    Article Snippet: Strategies to improve hematopoietic stem and progenitor cell (HSPC) mobilization from the bone marrow can have a pivotal role in addressing iatrogenic bone-marrow insufficiency from chemo(radio)therapy and overcoming peripheral blood stem cell transplantation (PBSCT) limitations such as insufficient mobilization.. Granulocyte-colony stimulating factor (G-CSF) represents the standard mobilization strategy for HSPC and has done so for more than three decades since its FDA approval.. Its association with non–G-CSF agents is often employed for difficult HSPC mobilization.

    Control:

    Article Title: Synergistic Effect of Human Chorionic Gonadotropin and Granulocyte Colony Stimulating Factor in the Mobilization of HSPCs Improves Overall Survival After PBSCT in a Preclinical Murine Model. Are We Far Enough for Therapy?
    Article Snippet: Strategies to improve hematopoietic stem and progenitor cell (HSPC) mobilization from the bone marrow can have a pivotal role in addressing iatrogenic bone-marrow insufficiency from chemo(radio)therapy and overcoming peripheral blood stem cell transplantation (PBSCT) limitations such as insufficient mobilization.. Granulocyte-colony stimulating factor (G-CSF) represents the standard mobilization strategy for HSPC and has done so for more than three decades since its FDA approval.. Its association with non–G-CSF agents is often employed for difficult HSPC mobilization.

    Bioprocessing:

    Article Title: Synergistic Effect of Human Chorionic Gonadotropin and Granulocyte Colony Stimulating Factor in the Mobilization of HSPCs Improves Overall Survival After PBSCT in a Preclinical Murine Model. Are We Far Enough for Therapy?
    Article Snippet: Strategies to improve hematopoietic stem and progenitor cell (HSPC) mobilization from the bone marrow can have a pivotal role in addressing iatrogenic bone-marrow insufficiency from chemo(radio)therapy and overcoming peripheral blood stem cell transplantation (PBSCT) limitations such as insufficient mobilization.. Granulocyte-colony stimulating factor (G-CSF) represents the standard mobilization strategy for HSPC and has done so for more than three decades since its FDA approval.. Its association with non–G-CSF agents is often employed for difficult HSPC mobilization.

    Suspension:

    Article Title: Laminin alpha 5 regulates mammary gland remodeling through luminal cell differentiation and Wnt4-mediated epithelial crosstalk
    Article Snippet: .. Single-cell suspension of isolated primary mouse mammary epithelial cells (MMECs) was resuspended in 0.2% BSA in Dulbecco's PBS, and the cells were incubated with the following primary antibodies for analysis: CD29-FITC (Miltenyi Biotech, 130-102-975); CD24-APC (M1/69, BioLegend, 101814); CD31-BV421 (MEC13.3, BD Biosciences, 562939); CD45-BV421 (30 F11, BD Biosciences, 563890); Ter119-BV421 (BD Biosciences, 563998); Sca1-BV711 (D7, BioLegend, 108131); and CD49b-PE (HMα2, BioLegend, 103506). ..



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    Characterization of SCAPs and DESCs. a Microscopic image of the apical buds from the lower incisors of 5-7-day-old C57BL/6 mice. b Primary mixed cell population. c Purified SCAPs exhibiting spindle-shaped morphology. d Multidirectional differentiation potential of SCAPs. Alcian Blue, Alizarin Red, and Oil Red O staining confirmed glycosaminoglycan deposition, mineralized nodule formation, and lipid droplet accumulation, indicative of chondrogenic, osteogenic, and adipogenic differentiation, respectively. e , f Flow cytometry analysis revealed CD24, CD133, and CD90 positivity, and CD45 negativity in SCAPs ( n = 3). The raw dot plots are presented in Supplementary Fig. S3. g Purified DESCs displaying cobblestone-like morphology. h Immunofluorescence imaging demonstrated positive K14 expression in DESCs (K14, green; DAPI, blue). i , j Flow cytometry analysis revealed <t>CD29</t> and CD49f positivity, and CD90 negativity in DESCs ( n = 3). The raw dot plots are presented in Supplementary Fig. S3
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    Characterization of SCAPs and DESCs. a Microscopic image of the apical buds from the lower incisors of 5-7-day-old C57BL/6 mice. b Primary mixed cell population. c Purified SCAPs exhibiting spindle-shaped morphology. d Multidirectional differentiation potential of SCAPs. Alcian Blue, Alizarin Red, and Oil Red O staining confirmed glycosaminoglycan deposition, mineralized nodule formation, and lipid droplet accumulation, indicative of chondrogenic, osteogenic, and adipogenic differentiation, respectively. e , f Flow cytometry analysis revealed CD24, CD133, and CD90 positivity, and CD45 negativity in SCAPs ( n = 3). The raw dot plots are presented in Supplementary Fig. S3. g Purified DESCs displaying cobblestone-like morphology. h Immunofluorescence imaging demonstrated positive K14 expression in DESCs (K14, green; DAPI, blue). i , j Flow cytometry analysis revealed <t>CD29</t> and CD49f positivity, and CD90 negativity in DESCs ( n = 3). The raw dot plots are presented in Supplementary Fig. S3
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    Characterization of SCAPs and DESCs. a Microscopic image of the apical buds from the lower incisors of 5-7-day-old C57BL/6 mice. b Primary mixed cell population. c Purified SCAPs exhibiting spindle-shaped morphology. d Multidirectional differentiation potential of SCAPs. Alcian Blue, Alizarin Red, and Oil Red O staining confirmed glycosaminoglycan deposition, mineralized nodule formation, and lipid droplet accumulation, indicative of chondrogenic, osteogenic, and adipogenic differentiation, respectively. e , f Flow cytometry analysis revealed CD24, CD133, and CD90 positivity, and CD45 negativity in SCAPs ( n = 3). The raw dot plots are presented in Supplementary Fig. S3. g Purified DESCs displaying cobblestone-like morphology. h Immunofluorescence imaging demonstrated positive K14 expression in DESCs (K14, green; DAPI, blue). i , j Flow cytometry analysis revealed <t>CD29</t> and CD49f positivity, and CD90 negativity in DESCs ( n = 3). The raw dot plots are presented in Supplementary Fig. S3
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    Image Search Results


    Characterization of SCAPs and DESCs. a Microscopic image of the apical buds from the lower incisors of 5-7-day-old C57BL/6 mice. b Primary mixed cell population. c Purified SCAPs exhibiting spindle-shaped morphology. d Multidirectional differentiation potential of SCAPs. Alcian Blue, Alizarin Red, and Oil Red O staining confirmed glycosaminoglycan deposition, mineralized nodule formation, and lipid droplet accumulation, indicative of chondrogenic, osteogenic, and adipogenic differentiation, respectively. e , f Flow cytometry analysis revealed CD24, CD133, and CD90 positivity, and CD45 negativity in SCAPs ( n = 3). The raw dot plots are presented in Supplementary Fig. S3. g Purified DESCs displaying cobblestone-like morphology. h Immunofluorescence imaging demonstrated positive K14 expression in DESCs (K14, green; DAPI, blue). i , j Flow cytometry analysis revealed CD29 and CD49f positivity, and CD90 negativity in DESCs ( n = 3). The raw dot plots are presented in Supplementary Fig. S3

    Journal: Stem Cell Research & Therapy

    Article Title: Lumican from DESC spheroids enhances odontoblastic differentiation of SCAPs

    doi: 10.1186/s13287-025-04849-7

    Figure Lengend Snippet: Characterization of SCAPs and DESCs. a Microscopic image of the apical buds from the lower incisors of 5-7-day-old C57BL/6 mice. b Primary mixed cell population. c Purified SCAPs exhibiting spindle-shaped morphology. d Multidirectional differentiation potential of SCAPs. Alcian Blue, Alizarin Red, and Oil Red O staining confirmed glycosaminoglycan deposition, mineralized nodule formation, and lipid droplet accumulation, indicative of chondrogenic, osteogenic, and adipogenic differentiation, respectively. e , f Flow cytometry analysis revealed CD24, CD133, and CD90 positivity, and CD45 negativity in SCAPs ( n = 3). The raw dot plots are presented in Supplementary Fig. S3. g Purified DESCs displaying cobblestone-like morphology. h Immunofluorescence imaging demonstrated positive K14 expression in DESCs (K14, green; DAPI, blue). i , j Flow cytometry analysis revealed CD29 and CD49f positivity, and CD90 negativity in DESCs ( n = 3). The raw dot plots are presented in Supplementary Fig. S3

    Article Snippet: For DESC analysis, cells were labeled with phycoerythrin (PE)-conjugated CD49f antibody (12-0495-82, Thermo Fisher Scientific, USA, 1:500), fluorescein isothiocyanate (FITC)-conjugated CD29 (11-0291-82, Thermo Fisher Scientific, USA, 1:500), and CD90 antibodies (ab25672, Abcam, UK, 1:500).

    Techniques: Purification, Staining, Flow Cytometry, Immunofluorescence, Imaging, Expressing